•35 year old healthy male
All
vegetables were triple-boiled in large amount of water
•
•Intake estimated. <25 ug/day vs. requirement of 200ug/day
•
•Supplements of all other B vitamins
•
•3
weeks – fall in serum folate
•
•7
weeks – white cells neutrophil hypersegmentation
•
•16
weeks - depression
& irritability
•
•17
weeks - fall in erythrocyte folate
•
•18-20 weeks - macrocytic red blood cells, fall in haemoglobin, bone marrow – megaloblastic anaemia